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Handling Cells, Culture Media and Cryopreservation
Biotechnology · Chapter 14 · 5. Introduction to Cell Culture Technology
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- Cell sorting by FACS; cell counting by haemocytometer; cell viability by trypan blue exclusion
- Nutrient media: LB, MS, White's, RPMI, DMEM and FBS
- Preparing culture medium: inorganic and organic nutrients, macro- and micronutrients, antibiotics, antimycotics, auxins and cytokinins
- Cryopreservation using liquid nitrogen