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Tools of Recombinant DNA Technology
Biotechnology · Chapter 5 · 2. Genetic Engineering
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Topics 5
- Concept of gene cloning and genetic engineering; restriction endonucleases (types) and other enzymes used in cloning
- Extraction and purification of DNA from bacterial, plant and animal cells
- Selection of prokaryotic and eukaryotic host cells
- Vectors: characteristics; plasmids pBR322 (two antibiotic-resistance genes) and pUC (lacZ gene)
- Cosmids, phages (M13 and lambda), YACs and BACs - stability and carrying capacity; viral vectors CaMV, retrovirus, SV40 (names only)