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Gene Transfer, Selection of Recombinants and DNA Libraries
Biotechnology · Chapter 6 · 2. Genetic Engineering
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- Vectorless gene transfer: transformation, transfection, electroporation, liposome-mediated transfer, microinjection, biolistics
- Vector-mediated transfer: Agrobacterium tumefaciens and the Ti plasmid
- Identifying recombinants: direct selection (green fluorescent protein) and insertional inactivation (blue-white selection, antibiotic resistance)
- Construction of a recombinant DNA molecule; genomic and cDNA libraries