Plant Tissue Culture and Its Applications

Biotechnology · Chapter 11 · 3. Cell Culture Technology

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Isolation of single cells (mechanical and enzymatic); synchronisation by starvation, inhibition and mitotic arrest
Cellular totipotency; differentiation, de-differentiation and re-differentiation
Haploid production (androgenesis, gynogenesis) and triploid production for seedless crops
In-vitro pollination; zygotic embryo culture and embryo rescue
Somatic hybridisation by protoplast fusion (pomato)
Micropropagation; virus-free plants; synthetic seeds; biodegradable plastics (PHB)