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Tools and Techniques of Cell Culture
Biotechnology · Chapter 9 · 3. Cell Culture Technology
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- Uses of laboratory instruments: centrifuge, laminar air flow hood, biosafety cabinet, pH meter, autoclave, vortex mixer, hot air oven, magnetic stirrer, balance, micro-filtration unit, incubators, inverted microscope, T-flasks
- Bioreactor: diagram, components and functions; stirred-tank and sparged types (brief)
- Sterilisation of the culture room, apparatus, transfer area, media, vitamins and living material
- Cryopreservation; cell counting by haemocytometer; viability by Evans blue; cell sorting by FACS
- Types of media (synthetic, semi-synthetic, natural); LB agar and broth, MS and White's media, RPMI, DMEM and FBS; nutrients, antibiotics, auxins and cytokinins; pH and solidifying agents