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Biotechnology: Principles and Processes
Biology · Chapter 14 · Unit 4: Biotechnology and its Applications
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Your path through this chapter
Visual lesson Beta
- Part 1: Tools of recombinant DNA technology17 slides
- Part 2: Getting genes into hosts and making products17 slides
Flashcards Beta
30 cards for quick recall of key terms and facts.
Review the cards Step 3: Not startedPractice
62 board-style questions with instant checks: Multiple choice 27, Short answer 15, Assertion and reason 5, Numerical 5.
Practise Step 4: Not startedChapter test Free
Short and long chapter tests in board pattern, with results and analysis.
Step 5: Not started- Chapter test (long) — Biotechnology: Principles and Processes · 40 marks · 100 min
- Chapter test (short) — Biotechnology: Principles and Processes · 20 marks · 50 min
Topics 6
- Definition and principles of biotechnology; isolation of genomic DNA by cell lysis; isolation of the gene of interest by gel electrophoresis
- Restriction enzymes: discovery, nomenclature, features (EcoRI, HindII); role of ligase; steps in making recombinant DNA
- Cloning vectors: features of a good vector; pBR322, Agrobacterium, retroviruses, BAC and YAC
- Transfer of rDNA into competent hosts (heat shock, microinjection, gene gun); selection of recombinants (antibiotic resistance, insertional inactivation, blue-white selection)
- Gene amplification in vivo and by PCR
- Bioreactors (stirred-tank and sparged-tank) and downstream processing